Journal: International Journal of Molecular Sciences

Article Title: Effect of Autophagy Regulated by Sirt1/FoxO1 Pathway on the Release of Factors Promoting Thrombosis from Vascular Endothelial Cells

doi: 10.3390/ijms20174132

Figure Lengend Snippet: ( A ) Viability of HUVEC treated with different concentrations of ox-LDL. ( B ) Concentration of von Willebrand factor (vWF) in media. ( C ) Expression of vWF on cytomembrane. ( D ) Expression of P-selectin on cytomembrane. ( E ) Expression of Sirt1, FoxO1, LC3-II/I and p62. Values are presented as the means ± SD (MTT and ELISA assay: n = 6, flow cytometry and Western Blot: n = 3). Compared with control groups at each time point, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Mouse anti-human Sirt1, FoxO1 and Rab7 antibodies and rabbit anti-human Ac-FoxO1 antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Effect of Autophagy Regulated by Sirt1/FoxO1 Pathway on the Release of Factors Promoting Thrombosis from Vascular Endothelial Cells

doi: 10.3390/ijms20174132

Figure Lengend Snippet: The viability of HUVEC treated with RSV ( A ) or SRT1720 ( B ). ( C ) Expression of Sirt1, FoxO1, Ac-FoxO1, LC3-II/I and p62 in HUVEC treated with RSV. ( D ) Expression of Sirt1, FoxO1, Ac-FoxO1, LC3-II/I and p62 in HUVEC treated with SRT1720. Values are presented as the means ± SD (MTT assay: n = 6; Western Blot: n = 3). Compared with the ox-LDL group, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Mouse anti-human Sirt1, FoxO1 and Rab7 antibodies and rabbit anti-human Ac-FoxO1 antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Expressing, MTT Assay, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Effect of Autophagy Regulated by Sirt1/FoxO1 Pathway on the Release of Factors Promoting Thrombosis from Vascular Endothelial Cells

doi: 10.3390/ijms20174132

Figure Lengend Snippet: Effect of gene silencing Rab7 on the inhibition of vWF and P-selectin secretion by Sirt1/FoxO1 pathway activation. HUVECs were transfected with Rab7 siRNA for 24 h and incubated with 100 μg/mL ox-LDL and 10 μM RSV or 5 μM SRT1720 for 12 h. The secretion of vWF to extracellular ( A ) was determined by ELISA assay, and the secretion of vWF ( B ) and P-selectin ( C ) to cytomembrane was detected by flow cytometry. Values are presented as the means ± SD (ELISA assay: n = 6, flow cytometry: n = 3). Statistical significance between the ox-LDL treated group and other groups was determined by one-way ANOVA: Compared with the ox-LDL group, *** p < 0.001.

Article Snippet: Mouse anti-human Sirt1, FoxO1 and Rab7 antibodies and rabbit anti-human Ac-FoxO1 antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Inhibition, Activation Assay, Transfection, Incubation, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Effect of Autophagy Regulated by Sirt1/FoxO1 Pathway on the Release of Factors Promoting Thrombosis from Vascular Endothelial Cells

doi: 10.3390/ijms20174132

Figure Lengend Snippet: Mechanism of Sirt1/FoxO1 pathway regulating autophagy to modulate the release of vWF and P-selectin (Arrow: activation/increase; T arrow: inhibition).

Article Snippet: Mouse anti-human Sirt1, FoxO1 and Rab7 antibodies and rabbit anti-human Ac-FoxO1 antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Activation Assay, Inhibition